Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.

摘要: Objective GRWD1 (glutamate-rich WD40 repeat containing 1) is a multifunctional protein involved in multiple cellular regulatory pathways, particularly those associated with cell growth control. represented as potential oncogene several cancers, however, the function and mechanism of development colon cancer are still unknown. Materials methods IHC was used to detect expression carcinoma tissues. CCK-8, colony formation, EdU were measure proliferation after knockdown overexpression. The distribution cycle analyzed by flow cytometry. effect on migration invasion wound healing transwell assays. Results Overexpression tissues pathological grading, tumor size, N stage, TNM poor survival. had high sensitivity specificity distinguishing from noncancerous tissues, might be served an independent prognosis patients. Knockdown significantly inhibited induced arrest more drug susceptibility, suppressed invasion. exhibited these oncogenic activities its regulation PCNA Ki67, Cyclin A2 B1, ABCC1 GSTP1, MTA1 MTA2. Conclusion may play activity overexpression malignant characteristics survival outcome carcinoma. target for future therapy.