Toxicological, Biochemical, and Histopathological Analyses Demonstrating That Cry1C and Cry2A Are Not Toxic to Larvae of the Honeybee, Apis mellifera
作者： Yuan-Yuan Wang , Yun-He Li , Zachary Y. Huang , Xiu-Ping Chen , Jörg Romeis
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摘要: The honey bee, Apis mellifera, is commonly used as a test species for the regulatory risk assessment of insect-resistant genetically engineered (IRGE) plants. In current study, dietary exposure assay was developed, validated, and to assess potential toxicity Cry1C Cry2A proteins from Bacillus thuringiensis (Bt) A. mellifera larvae; are produced by different IRGE crops. assay, which uses soybean trypsin inhibitor (SBTI) positive control bovine serum albumin (BSA) negative control, measure responses larvae high concentrations Cry2A. Survival reduced development delayed when were fed SBTI (1 mg/g diet) but unaffected BSA (400 μg/g), (50 or μg/g). enzymatic activities not altered their midgut brush border membranes (BBMs) damaged after being with diets containing BSA, Cry1C, Cry2A; however, increased BBMs contained SBTI. study confirms that have no acute at >10 times higher than those detected in pollen Bt
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