Evaluation of risk of splenosis during laparoscopic splenectomy in rat model.

摘要: Laparoscopic splenectomy (LS) is an alternative to open surgery. However, there a theoretic risk of splenosis and abdominal cavity dissemination splenic cells if the capsule broken, as seen by experimental evidence tumoral cell mobilization pneumoperitoneum. We evaluated features after operated via approach or under laparoscopic control in model rat. A total 65 Sprague-Dawley rats were distributed seven groups that included approach, CO2 pneumoperitoneum LS, wall lift LS with without graft. Splenic function was 90 day later through (1) scintigraphy Tc-labeled heat-damaged erythrocytes; (2) determination circulating “pitted” cells; (3) analysis distribution pulp peritoneal cavity. Scintigraphy did not show viable residual tissue any group splenectomy; activity fossa observed 40% animals grafts. Splenectomy increased “pit” count, but it reduced normal values Necropsy showed 100% Abdominal implants significantly more frequently than surgery (80% vs. 20% 30%; p<0.05). In addition, trocar site (n=3) (n=2), whereas no wound group. conclude rat may facilitate ruptured spills compared gas (open laparoscopic).