Liquid-chromatographic quantification of piracetam.

摘要: Piracetam, an analog of gamma-aminobutyric acid, absorbs maximally at 197 nm. Its molar absorptivity 208 nm and pH 4.5 is 3576 (SD 251) L . mol-1 cm-1, approximately 45% its Direct quantification piracetam in biological extracts complicated by the fact that many other compounds absorb between 190 220 due to carbon-nitrogen bonding. Chromatography methanol serum aqueous humor on a reversed-phase C-18 column developed isocratically with KH2PO4 (0.1 mol/L, 4.8) allows detection 0.2 mmol per liter. Under these conditions retention time about 5 min. The detector response linear for quantities 15 nmol. method rapid, inexpensive, convenient clinical laboratory.