Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field
作者： Gerard P. ‘t Lam , Jelmer A. van der Kolk , Akshita Chordia , Marian H. Vermuë , Giuseppe Olivieri
DOI: 10.1021/ACSSUSCHEMENG.7B00892
关键词:
摘要: Pulsed electric field (PEF) is considered to be a very promising technology for mild cell disruption. The application of PEF microalgae that have rigid wall, however, hampered by the presence outer wall. A wall free mutant C. reinhardtii was used mimic pretreated with removed investigate possibility using protein release from microalgae. complete hydrophilic proteins mutants observed whereas treatment on containing species resulted in substantially lower yields. Additional experiments showed even at low energy input (0.05 kWh/kgbiomass), still about 70% could released respect bead beating as reference. These were water-soluble while hydrophobic chlorophyll remained mainly entrapped particles. SEM-analysis these particles only opened cells, instead completely fragmen...
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