Community dynamics of a mixed-bacterial culture growing on petroleum hydrocarbons in batch culture.

摘要: The effects of various hydrocarbon substrates, and a chemical surfactant capable enhancing crude-oil biodegradation, on the community structure mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% identified at genus level or better by phospholipid fatty acid analysis over 7-day time course experiments. Cultures exposed to 20 g/L Bow River crude oil with without 0.625 Igepal CO-630 (a nonylphenol ethoxylate surfactant), 5 saturates, aromatics, 125 refinery sludge. A group six genera dominated cultures: Acinetobacter, Alcaligenes, Ochrobactrum, Pseudomonas/Flavimonas, Stenotrophomonas, Yersinia. Species from four shown be degradation, counts degrading total heterotrophic bacteria nearly identical. Pseudomonas/Flavimonas Stenotrophomonas normally during early portions cultures, although lag phase appears have been increased addition. Acinetobacter calcoaceticus was most frequently isolated microorganism exposure saturate fraction oil. Regardless substrate, culture medium supported greater variety organisms latter cultures. Understanding dynamics mixed bacterial cultures involved treatment heterogeneous waste substrates may assist process development optimization studies.