Downregulation of miR-145 contributes to lung adenocarcinoma cell growth to form brain metastases
作者： CHUNYANG ZHAO , YAN XU , YONGQIANG ZHANG , WEIWEI TAN , JIANXIN XUE
DOI: 10.3892/OR.2013.2728
关键词:
摘要: The development of metastases involves the dissociation cells from primary tumor, penetrating basement membrane, invasion and exiting vasculature to seed, finally colonizing in distant tissues. formation brain metastasis (BM) lung adenocarcinoma remains poorly understood. We examined differential microRNA (miRNA) expression profiles 5 3 metastatic samples by Agilent miRNA Microarrays. Five upregulated miRNAs (miRs-9*, -1471, 718, 3656, 720) downregulated (miRs-214, -145 -23a) were detected. 4 most significantly deregulated (miR-145, miR-214, miR-9* miR-1471) validated additional 43 (35 8 samples) using TaqMan quantitative PCR. By functional assay, we found that miR-145 can regulate ability proliferation A549 SPC-A1 vitro, but is not related lymph node metastasis, migration invasion. These results suggest may have a cell type-specific function play important roles process BM adenocarcinoma.
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