Characterization of a mitotic mutant of durum wheat.

摘要: An ethyl methanesulfonate-induced mitotic mutant of durum wheat (Triticum turgidum L. var. durum; 2n=4x=28) was found. We have characterized the to determine mechanism abnormal cell division and test for temperature effects on division. Stained root-tip meristems pollen mother cells were studied with brightfield, phase contrast, immunofluorescence microscopy. Abnormal included metaphase a multiple normal complement (8x=56, or 16x=112), multinucleate cells, 4C, 8C, 16C mononucleate exhibiting incomplete cytokinesis. The had three classes cells: euploid bivalent pairing, multiploid multivalent pairing. Preprophase bands spindles in cells. Some asymmetrical phragmoplasts phragmoplast dismantling that produced Failure cytokinesis followed by nuclear fusion mechanisms To sensitivity mutant, seedlings germinated under six different regimes. As germination increased, frequency increased. When crossed as female wheat, 3% hybrids hexaploid, indicating functional–unreduced gametes formed megaspores.