Behavior of Microtubules in Living Plant Cells.

摘要: Cytoplasmic MTs are recognized as important elements in plant development. They categorized four principal arrays: the PPB, MA, phragmoplast, and cortical array, they appear to participate fundamental aspects of cell division, growth, differentiation. Thus, such diverse activities prediction alignment plate, movement chromosomes transport plate vesicles, orientation cellulose wall microfibrils all mediated by varying degrees. Our knowledge about distribution function has been gleaned largely from studies at electron microscope level, more recently light level using immunofluorescence methods. Because most these observations have based on cells that fixed, it not possible examine directly dynamic properties behavior MTs. Transitional states inferred looking numerous isolated examples, which then placed into a sequence. Other aspects, sensitivity drugs, ions, or cold temperature, also approximated through fixed periodic intervals following application an agent. Some MT behavior, including, notably, their turnover, beyond reach indirect To enlarge our understanding MTs, we introduced technique fluorescent analog cytochemistry applied (Zhang et al., 1990b). In this procedure fluorescently labeled exogenous tubulin, is injected cell, incorporates endogenous pool acts reporter. observed with CLSM, can be examined under any experimental condition exposed. The results produced new information transitions live cells, address Update. including identification organizing centers, tubulin isoforms, MAPs considered other recent topical reviews (Lambert, 1993; Cyr, 1994; Goddard Shibaoka, Cyr Palevitz, 1995; Joshi 1996), so will covered here. INCORPORATION OF HETEROLOGOUS ANIMAL TUBULIN INTO PLANT MTS; OBSERVATIONS BY CLSM