Standardization of an orthotopic mouse brain tumor model following transplantation of CT-2A astrocytoma cells.

摘要: Animal models of glial-derived neoplasms are needed to study the biological mechanisms glioma tumorigenesis and those that sustain disease state. With aim develop characterize a suitable in vivo experimental mouse model for infiltrating astrocytoma, with predictable reproducible growth patterns recapitulate human this was undertaken analyze long-term course syngeneic orthotopically implanted CT-2A astrocytoma C57BL/6J mice. Intracranial injection cells into caudate-putamen resulted development an aggressive tumor showing typical features glioblastoma multiforme, sharing close histological, immunohistochemical, proliferative, metabolic profiles. To simulate metastatic brain, were injected through internal carotid artery. Tumors identical obtained by intracranial obtained. Finally, re-isolated from brain tumors transcranially re-injected healthy These generated new indistinguishable initial ones, suggesting self-renewal cells. Small-animal essential testing novel therapies directed against relevant molecular targets. In preliminary study, chronically treated small molecule 77427, gastrin-releasing peptide (GRP) blocker compound inhibits angiogenesis. Treated animals developed significantly smaller than controls, antitumor action 77427 glioblastomas. We conclude orthotopic model, as described herein, is appropriate explore preclinical trials promising drugs.