Metallacarboranes on the Road to Anticancer Therapies: Cellular Uptake, DNA Interaction, and Biological Evaluation of Cobaltabisdicarbollide [COSAN]−
作者： Isabel Fuentes , Tania García-Mendiola , Shinichi Sato , Marcos Pita , Hiroyuki Nakamura
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摘要: After uptake by U87 MG and A375 cancer cells, cobaltabisdicarbollide [COSAN]- distributes between membrane nucleus presents no relevant cytotoxicity against both cell lines even for long incubation times. The of Na[COSAN] was also tested towards one normal line, the V79 fibroblasts, in order to ascertain noncytotoxic profile compound. As cell's contains DNA, interaction double-stranded calf thymus DNA (CT-dsDNA) has been investigated. There is a strong molecules forming nanohybrid CT-dsDNA-[COSAN] biomaterial, which fully characterized. Moreover, shows characteristic redox peaks ascribed oxidation/reduction Co3+/2+ at formal potential -1.444 V it can be accumulated surface-immobilized layer glassy carbon electrodes. equilibrium surface-binding constants (Kox /Kred ), confirm that interacts with an intercalative or electrostatic mode, depending on ionic strength solution, were estimated. In addition, high binding affinity proteins observed 11 B{1 H} NMR confirmed vivo. Finally, biodistribution studies mice run. administration, distributed into many organs but mainly reticuloendothelial system (RES), including liver spleen. 1 h, formation aggregates plasma protein plays role profile; accumulate mostly lungs. Na[COSAN], displays low toxicity cells accumulating boron within nucleus, could act as suitable compound further developments neutron capture therapy (BNCT) agents.
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