Vanadate raises fructose 2,6-bisphosphate concentrations and activates glycolysis in rat hepatocytes.

摘要: In rat hepatocytes, vanadate increases fructose 2,6-bisphosphate (Fru-2,6-P2) in a time- and dose-dependent manner, counteracts the decrease this metabolite caused by glucagon, forskolin or exogenous cyclic AMP. Vanadate does not directly modify activity of 6-phosphofructo-2-kinase, even though it can counteract inactivation enzyme glucagon. Furthermore, raises yield 3H2O from [3-3H]glucose, indicating that flux through 6-phosphofructo-1-kinase. Moreover, hepatocytes incubated presence glucose production both lactate CO2. Therefore has insulin-like effects on glycolytic pathway hepatocytes. These results clearly contrast with our previous observation exerts glycogenolytic non-insulin-like glycogen synthase phosphorylase.