Bacterial Infection Affects Protein Synthesis in Primary Lymphoid Tissues and Circulating Lymphocytes of Rats

摘要: Bacterial infection alters whole-body protein homeostasis. Although immune cells are of prime importance for host defense, the effect sepsis on their synthesis rates is poorly documented. We analyzed in rat primary lymphoid tissues and circulating lymphocytes after infection. Male Sprague-Dawley rats were studied 1, 2, 6 or 10 d an intravenous injection live Escherichia coli. Control healthy consumed food ad libitum (d 0) pair-fed to infected rats. Protein was quantified using a flooding dose L-(4,4,4- 2 H3)valine. Sepsis induced delayed increase total blood leukocytes rapid persistent inversion counts. Basal fractional (ks) 117, 73 29%/d bone marrow, thymus lymphocytes, respectively. Pair-feeding strongly depressed absolute (ASR) marrow 10) 2-10). The per se increased lymphocyte ks but at various postinfection times. It decreased 1) 1 2) ASR ASR. Our results reflect deleterious anorexia tissues. defense against bacterial exhibited time- tissue-dependent modifications synthesis, indicating that data not representative system as whole. Optimization nutritional supports would be facilitated by including measurements system. J. Nutr. 132: 2028 -2032, 2002.