Molecular cloning of the avian myelocytomatosis virus genome and recovery of infectious virus by transfection of chicken cells.

摘要: The avian retrovirus myelocytomatosis virus 19 (MCV) possesses an interesting diversity of oncogenic potentials, but the has proven difficult to study because its inability replicate without assistance a helper virus. We have therefore isolated and amplified genome MCV by molecular cloning in procaryotic vector. topography cloned DNA was explored use restriction endonucleases radioactive complementary DNAs representing specific domains genomes. appeared be authentic representation genome: size genetic were comparable those MCV, transfection into chicken cells (in company with suitable virus) gave rise transforming potentials MCV. availability should facilitate variety biochemical manipulations directed at elucidating mechanism oncogenesis