Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.)

摘要: Abstract The effect of sucrose concentration on callus induction followed by differentiation embryogenic derived from petal explants four carnation cultivars (Nelson, Sagres, Spirit and Impulse) was investigated. Embryogenic calli were produced Murashige Skoog [Murashige, T., Skoog, F.A., 1962. Revised medium for rapid growth bioassays with tobacco tissue cultures. Physiol. Plant 154, 73–479] basal (MS) culture containing six concentrations (3, 6, 9, 12, 15 18%, w/v) all supplemented 9 μM 2,4-dichlorophenoxyacetic acid (2,4-D) 0.8 μM 6-benzyladenine (BA). Maximum frequency obtained the media 9 12% sucrose. Somatic embryos induced a hormone-free MS seven Development somatic enhanced increasing 1.5 to 12%, while it reduced in higher 18%. However, normal not developed 3% Ninety-five percent regenerated form entire plantlets when they transferred onto half-strength Plantlets also continued grow normally under greenhouse condition.