Control of in vitro somatic embryogenesis of the spindle tree ( Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium
作者： A. Biahoua , L. Bonneau
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摘要: In vitro somatic embryogenesis was achieved from zygotic embryo explants of a woody angiosperm species, the spindle tree, cultivated on various culture media differing in their sugar type and concentration, or applied osmotic potential. The highest frequency obtained with 350 mM sucrose, 89 glucose concentration medium. Experiments only potential indicated that minimal threshold is required to stimulate emergence embryos. Elevated concentrations have an inhibitory effect, independent while elevated sucrose mainly act osmotically, stimulating numerous
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