Embryonic stem cell extracts improve wound healing in diabetic mice

摘要: Impaired wound healing significantly impacts morbidity and mortality in diabetic patients, necessitating the development of novel treatments to improve process. We here investigated topical use acellular embryonic stem cell extracts (EXTs) db/db mice. Wounds were induced mice, which subsequently treated with EXTs, 3T3 fibroblast line protein (3T3XTs) or saline as a control. Pathology mechanistic assays then performed. The vivo administration EXTs facilitates closure, contraction re-epithelialization. Moreover, reduced number wound-infiltrating CD45+ inflammatory cells increased rate repair angiogenesis compared controls. Interestingly, EXT effect was partly enhanced by collagen-based biocompatible scaffold. In vivo, percentage regulatory T wounded tissue, while vitro treatment cell-mediated IFN-γ production. Proteomic screening revealed 82 proteins differentially segregating extracts, APEX1 identified key player for observed immunomodulatory EXTs. are endowed immunoregulatory anti-inflammatory properties; their improves preclinical models.