Intrapulmonary administration of bone-marrow derived M1/M2 macrophages to enhance the resolution of LPS-induced lung inflammation: noninvasive monitoring using free-breathing MR and CT imaging protocols

摘要: Alveolar macrophages, with their high functional plasticity, were reported to orchestrate the induction and resolution of inflammatory processes in chronic pulmonary diseases. Noninvasive imaging modalities that offer simultaneous monitoring inflammation progression tracking macrophages subpopulations involved cascade, can provide an ideal specific diagnostic tool visualize action mechanism its initial stages. Therefore, purpose current study was evaluate role M1 M2 lipopolysaccharide (LPS)-induced lung monitor this process using noninvasive free-breathing MRI CT protocols. Bone-marrow derived first polarized then labeled superparamagnetic iron oxide nanoparticles. BALB/c mice received intrapulmonary instillation these ex vivo or macrophages. The biodistribution subsequent noninvasively monitored micro-CT. Confirmatory immunohistochemistry analyses performed on tissue sections macrophage markers. As expected, large areas imaged MR micro-CT observed within lungs following LPS challenge. Subsequent administration resulted a significant decrease starting from 72 h. established reduction both subsets. An enhanced compared M1. demonstrated decreased LPS-induced inflammation. protocols enabled efficient