Effect of reducing agents on the acidification capacity and the proton motive force of Lactococcus lactis ssp. cremoris resting cells.

作者: Yves Waché , Christophe Riondet , Charles Diviès , Rémy Cachon

DOI: 10.1016/S1567-5394(02)00051-8

关键词:

摘要: Abstract Reducing agents are potential inhibitors of the microbial growth. We have shown recently that dithiothreitol (DTT), NaBH 4 and H 2 can modify proton motive force resting cells Escherichia coli by increasing membrane protons permeability [Eur. J. Biochem. 262 (1999) 595]. In present work, effect reducing on Lactococcus lactis ssp. cremoris , a species widely employed in dairy processes was investigated. DTT did not affect acidification nor ΔpH, contrast to previously reported E. . The Δ Ψ slightly increased (30 mV) at low pH (pH 4) presence 31 mM or 2.6 case Na S O small amounts (0.9 mM) drastically decreased range this product abolish ΔpH. These results discussed terms diversity action chemical reagents strain sensitivity.

参考文章(33)
R Otto, R G Lageveen, H Veldkamp, W N Konings, Lactate efflux-induced electrical potential in membrane vesicles of Streptococcus cremoris. Journal of Bacteriology. ,vol. 149, pp. 733- 738 ,(1982) , 10.1128/JB.149.2.733-738.1982
E. R. Kashket, T. H. Wilson, Role of Metabolic Energy in the Transport of β-Galactosides by Streptococcus lactis1 Journal of Bacteriology. ,vol. 109, pp. 784- 789 ,(1972) , 10.1128/JB.109.2.784-789.1972
Klaus G. STEINHÄUSER, Paul WOOLLEY, Bernd EPE, Jan DIJK, Structure of Ribosomal Protein L6 from Escherichia coli FEBS Journal. ,vol. 127, pp. 587- 595 ,(2005) , 10.1111/J.1432-1033.1982.TB06913.X
D. H. Park, M. Laivenieks, M. V. Guettler, M. K. Jain, J. G. Zeikus, Microbial Utilization of Electrically Reduced Neutral Red as the Sole Electron Donor for Growth and Metabolite Production Applied and Environmental Microbiology. ,vol. 65, pp. 2912- 2917 ,(1999) , 10.1128/AEM.65.7.2912-2917.1999
Mireille Yvon, Emilie Chambellon, Alexander Bolotin, Florence Roudot-Algaron, Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763. Applied and Environmental Microbiology. ,vol. 66, pp. 571- 577 ,(2000) , 10.1128/AEM.66.2.571-577.2000