Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus

摘要: Adult Paragonimus heterotremus were recovered from the lungs and pleural cavity of cats orally infected with metacercariae. The worms ground extracted distilled water. soluble crude antigen (CA) contained about 40% proteins which could be fractionated by gel filtration on Sephadex G-200 into three profiles namely F1, F2 F3. CA its used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to P. groups patients, i.e. patients whose sputum and/or faeces revealed eggs (group 1), other parasitic infections 2), bacterial proven tuberculosis 3) healthy, parasite-free controls 4). sensitivity specificity when F1 was as 100%. Western blot analysis that specific a non-protein component Mr35 kDa.