Quantification of Wilms' tumor 1 mRNA by digital polymerase chain reaction.
作者： Yuki Koizumi , Daisuke Furuya , Teruo Endo , Kouichi Asanuma , Nozomi Yanagihara
DOI: 10.1007/S12185-017-2336-8
关键词:
摘要: Wilms’ tumor 1 (WT1) is overexpressed in various hematopoietic tumors and widely used as a marker of minimal residual disease. WT1 mRNA has been analyzed using quantitative real-time polymerase chain reaction (real-time PCR). In the present study, we 40 peripheral blood bone marrow samples obtained from cases acute myeloid leukemia, lymphoblastic myelodysplastic syndrome at Sapporo Medical University Hospital April 2012 to January 2015. We performed quantification was QuantStudio 3D Digital PCR System (Thermo Fisher Scientific) compared results between digital technology. The correlation very strong (R = 0.99), detection limits two methods were equivalent. able accurately detect lower WT levels with PCR. technology can thus be utilized predict WT1/ABL1 expression level should useful for diagnosis or evaluation drug efficiency patients leukemia.
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