Protein kinase C-mediated phosphorylation in intact cells
作者： Lee A. Witters , Perry J. Blackshear
DOI: 10.1016/0076-6879(87)41087-2
关键词:
摘要: Publisher Summary The calcium- and phospholipid-dependent protein kinase (protein C) is widely distributed in the tissues of mammals other organisms. Diacylglycerol greatly increases affinity for both calcium phospholipid. Agonist-induced turnover membrane inositol phospholipid taken as evidence potential C activation. Based on this criterion alone, activation suggested to be involved signal transducing cascade several agonists, such thyrotropin-releasing hormone. This chapter describes application four techniques that are applied a variety cell systems address these criteria. These include use phorbol esters diacylglycerols modulate intracellular C, description ubiquitous phosphoprotein serves an important reporter intact cells, creation cells deficient assay permits measurement activity crude tissue extracts.
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