Vinculin phosphorylation in response to calcium and phorbol esters in intact cells.

摘要: Vinculin phosphorylation in both chick embryo fibroblasts and Swiss 3T3 cells was increased by either calcium or biologically active phorbol esters. Increased of vinculin noted as early 10 min following 12-myristate 13-acetate treatment maximal at about 1 h. Maximal increases were approximately 100 nM 13-acetate. Phorbol 12,13-dibutyrate (80 nM), a less potent ester, resulted smaller than equimolar concentrations. Phorbol, dibutyryl cAMP, cGMP had no significant effect on phosphorylation. No correlation found between the morphological changes induced Tryptic peptide analysis revealed multisite Phosphorylation only three peptides significantly treatment. Phosphoamino acid serine threonine residues. The low level phosphotyrosine present control not These findings combined with studies purified protein kinase C (Werth, D. K., Niedel, J. E., Pastan I. (1983) Biol. Chem. 258, 11423-11426) suggest hypothesis that may be involved regulation vinculin, cytoskeletal protein.