Effect of lipd geometry on vesicle fusion kinetics in an artificial cell model for exocytosis

摘要: The process of stimulated exocytosis is fundamental to neuronal communication. This involves fusion neurotransmitter-filled vesicles with the plasma membrane presynaptic terminal, diffusive transport across synaptic cleft and recognition binding receptors on postsynaptic surface. thought go via formation a number intermediates structures described by stalk theory. It results in pore or nanotube, which then expands incorporate vesicular material surrounding membrane. In this thesis we describe use model system for studying effect lipid composition dynamics final stages exocytotic fusion. Surface-immobilized multilamellar liposomes created from soy bean extract dehydration/rehydration were manipulated into forming tubular structure inside unilamellar liposome. tube was brought inflate vesicle, fused released contained catechol solution. By varying lipids substitution either 30%phosphatidylcholine 30% phosphatidylethanolamine wanted investigate different geometries vesicle kinetics during exocytosis. Detection release profiles done amperometry. Although more experiments are needed confirm presented here, data so far suggest that increase phosphatidylcholine affect increasing rate chemical release. However, amount slight speed detected but not concluded be significantly control measurements.