Genetic analysis using genomic representations

摘要: Analysis of the genetic changes in human tumors is often problematical because presence normal stroma and limited availability pure tumor DNA. However, large amounts highly reproducible “representations” genomes can be made by PCR from nanogram restriction endonuclease cleaved DNA that has been ligated to oligonucleotide adaptors. We show here representations are useful for many types analyses, including measuring relative gene copy number, loss heterozygosity, comparative genomic hybridization. Representations may prepared even sorted nuclei fixed archived biopsies.