Immunohistochemistry detection method

作者: Lars Winther , Susanne Gabs , Kenneth Heesche Petersen , Jesper Lohse

DOI:

关键词:

摘要: The invention provides compositions and methods for the detection of targets in a sample; particular, an immunohistochemistry (IHC) sample. Probes detectable labels may be provided multiple layers order to increase flexibility system, allow amplification enhance signal from target. created by incorporating probes into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases degenerate base pairing schemes. are compatible with (IHC), but also could used immunocytochemistry (ICC), situ hybridization (ISH), flow cytometry, enzyme immuno-assays (EIA), linked (ELISA), blotting (e.g. Western, Southern, Northern), labeling inside electrophoresis systems or on surfaces arrays, precipitation, among other general assay formats. is many different types targets, probes, labels.

参考文章(38)
Michael Egholm, Peter Eigil Nielsen, Rolf Hendrik Berg, Ole Buchardt, The use of nucleic acid analogues in diagnostics and analytical procedures ,(1992)
Ole Buchardt, Michael Egholm, Peter E. Nielsen, Rolf H. Berg, Histidine-containing peptide nucleic acids ,(1999)
Michael Egholm, Caifu Chen, Multipartite high-affinity nucleic acid probes ,(2001)
Kai Qin Lao, Timothy G. Geiser, Neil A. Straus, Analog probe complexes ,(2005)
Peter E Nielsen, Pierre Lagriffoul, Ole Di Buchardt, Peptide nucleic acid incorporating a chiral backbone ,(1995)
Christine Peponnet, Anton Simeonov, Anne R. Kofp-Sill, Michael R. Knapp, Systems and methods for high throughput genetic analysis ,(2002)
Antonius Lobberding, Burkhard Mielke, Eckhard Schwenner, Christoph Schwemler, Udo Stropp, Thorsten Potter, Wolfgang Springer, Axel Kretschmer, Nucleic acid-binding oligomers possessing C-branching for therapy and diagnostics ,(1994)
Michael Egholm, Peter Eigil Nielsen, Rolf Henrik Berg, Christopher John Stanley, Ole Buchardt, Use of nucleic acid analogues in the inhibition of nucleic acid amplification ,(1993)