Regulation of initiation factors during translational repression caused by serum depletion. Covalent modification.
作者： R Duncan , J W Hershey
DOI: 10.1016/S0021-9258(18)89049-4
关键词:
摘要: One to 2 h after transfer of HeLa cells into fresh serum-containing medium, when translation rates are maximal, the initiation factor proteins were examined on immunoblots two-dimensional gels. Eukaryotic (eIF)-2 alpha, eIF-2 beta, and eIF-4A each formed a single immunoreactive spot; gamma spots; eIF-4B complex array 12-20 spots. After 4 days growth in unreplenished have dropped 4-6-fold, several alterations isoelectric forms observed: alpha now occurred forms, beta was present 3-4 most acidic cluster variants decreased or absent while new variant appeared at basic end array. No changes observed for eIF-4A. The 35-50-kDa subunits multiprotein eIF-3 also showed no aforementioned states compared. Resolution 32P-labeled lysates by focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that modification loss reflected phosphorylation states. Stimulation 4-day grown with medium caused reversal modifications back prevailing shortly replating. This analysis indicates covalent appear concurrently decreasing suggests they may be causative.
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