Tissue-specific expression and thyroid hormone regulation of the endogenous placental growth hormone variant and chorionic somatomammotropin genes in a human choriocarcinoma cell line.

摘要: Human (h) placenta-derived choriocarcinoma cell lines (BeWo, JAR, and JEG-3) were examined for expression of pituitary GH (hGH-N) as well placental variant (hGHV) chorionic somatomammotropin (hCS, encoded by the hCS-A or hCS-B gene). RNA was isolated assessed using hGH-N complementary DNA since hGH hCS genes share more than 90% sequences similarity. The relative is BeWo > JAR JEG-3. In Be Wo cells of” hCS-A, hCS-B, hGH-V genes, but not hGH-N, observed polyadenylated oligonucleotide probes specific different family members. absence hGHN in due to deletion gross rearrangement gene. No difference seen between hGH/hCS genomic from these placenta when analyzed restriction digestion blotting. Treatment with 10 nM T3 results a 6-fold increase messenger members gene...