Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules.

摘要: The importance of the species different domains class I MHC molecules in peripheral T cell recognition and positive negative selection was evaluated a single system. In transgenic mice expressing AAD (containing alpha1+alpha2 HLA-A2.1 alpha3 domain H-2Dd), CTL response to influenza peptide M1(58-66) context as strong influenza-specific H-2Db-restricted response. However, this only discernible if target molecule also contained murine domain. contrast, about 30-fold weaker, these were indifferent origin Further analysis suggested that major impact transgene product enhance low affinity population AAD-restricted cells, presumably through species-specific interaction with CD8. Surprisingly, non-self human determinants not augmented mice, indicating cells selected are narrowly focused on AAD-related structures. indicated alphal+alpha2 well influenced magnitude determinants, effect mapped alpha2. We suggest alpha2 contain conserved structural elements augment avidity cell-class interactions, is particularly important molecules.