Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway

摘要: PURPOSE To investigate the cytotoxic activity of suramin against HepG2 human HCC cell line. METHODS cells were treated with 15, 30 and 45 μM suramin. proliferation was measured by MTT lactate dehydrogenase (LDH) assays. Heparan sulfate proteoglycans (HSPGs), glucuronic acid glucosamine levels measured. Moreover, apoptosis assessed measuring caspase-8 caspase-9 activities. The effect on compared same doses a standard drug, cisplatin. RESULTS Suramin blocked heparanase leading to dose-dependent increase in HSPGs reduction levels. reduced survival showed cytotoxicity manner LD50 45.04 μM, cisplatin (LD50 28.9 μM) (p<0.05). able but not caspase-8. CONCLUSION possesses properties, which can be partially explained its ability inhibit restore HSPGs. activates intrinsic without affecting extrinsic pathway.