Quick and simple method for increasing the reduced albumin fraction in human serum albumin preparations by using stronger neo-minophagen C
作者： Masahiro Sakata , Takumi Kawaguchi , Eitaro Taniguchi , Mitsuhiko Abe , Hironori Koga
DOI: 10.1111/J.1872-034X.2011.00863.X
关键词:
摘要: AIM Serum albumin exists in both oxidized and reduced forms. Reduced shows higher antioxidative effects; however, the percentage of is low human serum (HSA) preparation. Stronger neo-minophagen C (SNMC) containing cysteine, a nucleophilic amino acid, has potential to control redox state albumin. The aim this study was develop method for increasing fraction HAS preparation using SNMC. METHODS Human preparations were purchased from four different manufacturers. As reducing agent, SNMC (50, 100, or 200 µL) added 62.5 mg each HSA preparation, mixture incubated at room temperature 10-480 min. percentages determined by high-performance liquid chromatography. RESULTS significantly increased 15 min after treatment with 200 µL (manufacturer A; 27.7 ± 0.18% vs. 78.7 ± 0.36%, P < 0.01), dose-dependent relationship observed between increase dose Similar results obtained other three preparations. reached its peak mixing an then gradually decreased duration, irrespective CONCLUSIONS We devised also time-and dose-differences effect on HSA.
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