Anaerobic central metabolic pathways active during polyhydroxyalkanoate production in uncultured cluster 1 Defluviicoccus enriched in activated sludge communities.

摘要: A glycogen nonpolyphosphate-accumulating organism (GAO) enrichment culture dominated by the Alphaproteobacteria cluster 1 Defluviicoccus was investigated to determine metabolic pathways involved in anaerobic formation of polyhydroxyalkanoates, carbon storage polymers important for proliferation microorganisms enhanced biological phosphorus removal processes. FISH-microautoradiography and post-FISH fluorescent chemical staining confirmed acetate assimilation as polyhydroxyalkanoates under conditions. Chemical inhibition glycolysis using iodoacetate, isocitrate lyase 3-nitropropionate itaconate, indicated that is likely be channelled through both glyoxylate cycle Defluviicoccus. The effect inhibitors aconitase (monofluoroacetate) succinate dehydrogenase (malonate) suggested aconitase, but not dehydrogenase, active, providing further support role these GAOs. Metabolic fumarate reductase oxantel decreased polyhydroxyalkanoate production. This reduction operation reductive branch tricarboxylic acid cycle, which possibly production polyhydroxyvalerate component observed cultures. These findings were integrated with previous models GAOs enabled an central pathway model proposed.