Fabrication of two-layered channel system with embedded electrodes to measure resistance across epithelial and endothelial barriers.
作者： Nicholas J. Douville , Yi-Chung Tung , Ran Li , Jack D. Wang , Mohamed E.H. El-Sayed
DOI: 10.1021/AC9029345
关键词:
摘要: This manuscript describes a straightforward fabrication process for embedding Ag/AgCl electrodes within two-layer poly(dimethylsiloxane) (PDMS) microfluidic chip where an upper and lower channel are separated by semiporous membrane. system allows the reliable real-time measurement of transendothelial transepithelial electrical resistance (TEER), accepted quantification cell monolayer integrity, across cells cultured on membranes inside microchannels using impedance spectroscopy. The technique eliminates need costly or specialized microelectrode fabrication, enabling commercially available wire to easily be incorporated into PDMS microsystems measuring TEER under environments. capability confluent is confirmed (i) brain-derived endothelial (bEND.3), (ii) Madin Darby Canine Kidney Cells (MDCK-2), mouse myoblast (C2C12) (all from ATCC, Manassas, VA). values as function type culture time were measured both agree with previously published macroscale techniques. opens new opportunities conveniently resolving monitor in cultures.
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