Human keratinocytes and monocytes release factors which regulate the synthesis of major acute phase plasma proteins in hepatic cells from man, rat, and mouse.

摘要: Human keratinocytes and activated monocytes produces factors which can stimulate the proliferation of thymocytes. The same activity has also been implicated in regulating expression plasma proteins liver cells during acute phase reaction. To assess whether produced by such directly influence to change production proteins, we studied tissue culture response pattern hepatic from three species: human hepatoma ( HepG2 cells), primary cultures rat mouse hepatocytes. Conditioned media squamous carcinoma COLO-16 cells, normal epidermal peripheral were able synthesis specific proteins: alpha 1-antichymotrypsin HepG -2 1-antichymotrypsin, 1-acid glycoprotein, 1-acute protein, 2-macroglobulin hepatocytes, haptoglobin, hemopexin Only dexamethasone was found have further enhancing effect. increased could be explained an elevated level functional mRNA. Comparing thymocyte-stimulating activities with effects on protein production, some difference both between conditioned monocytes, responses cell systems. Furthermore, gel chromatography resulted partial separation Since there is no strict correlation thymocyte- hepatocyte-stimulating activities, presence different sets assumed.