Vascular repair utilising immobilised heparin conjugate for protection against early activation of inflammation and coagulation

摘要: Ischaemia-reperfusion injury (IRI) poses a major challenge in many thrombotic conditions and whole organ transplantation. Activation of the endothelial cells shedding protective vascular glycocalyx during IRI increase risk innate immune activation, cell infiltration severe thrombus formation, promoting damage to tissue. Here, we present novel one-step strategy protect vasculature by immobilisation unique multi-arm heparin conjugate endothelium. Applying new vitro blood chamber model, was found bind not only primary human but also directly collagen which adhered. Incubation hypoxic with freshly drawn chambers elicited coagulation activation reflected thrombin anti-thrombin formation binding platelets neutrophils. Immobilisation created coating, leading significant reduction recruitment compared untreated cells. This approach immobilising conjugates on basement membrane ensures endothelium against disorders