Antioxidant Profile of 1-Monocaffeoyl Glycerol in Lipophobic/Lipophilic Media.
作者： Longmei Weng , Lin Li , Lili Ji , Di Zhao , Zhenbo Xu
关键词:
摘要: Oxidative stress has been generally considered as one trigger of organism imbalance, resulting in lipid peroxidation, DNA damage and protein oxidation, which could be relieved by antioxidant supplement or endogenous system. In present study, 1-monocaffeoyl glycerol (1-MCG), an amphipathic caffeic acid natural derivative, was enzymatically synthesized Lipozyme 435, its profile both lipophilic lipophobic media evaluated. The 1-MCG identified HPLC-UV, HPLC-ESI-MS, 1 H/13 C-NMR. Subsequently, assays (DPPH assay) (ABTS, ORAC, erythrocyte hemolysis, ROS, MDA, GPx assays) systems were explored. better lasting DPPH· ABTS+· inhibitions than (CA) related to solubilities ethanol/water electron transfer ability. ORAC results suggested the radical scavenging activities (5 40 µM) higher Trolox. Furthermore, effectiveness against AAPH-induced erythrocytes oxidation indicated that can effectively inhibit hemolysis. ESEM also applied verify hemolysis inhibition morphology preservation abilities 1-MCG. Besides, showed able prevent ROS from invasion, reduce production up-regulated activity, terminate maintain integrity structure function erythrocytes. PRACTICAL APPLICATION: As amphiphilic synthesized, purified, identified. 1-Monocaffeoyl significantly eliminate radicals including DPPH·, , AAPH ethanol, water, PBS system, respectively. protect induced
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