Sandwich-type electrochemical immunosensor using dumbbell-like nanoparticles for the determination of gastric cancer biomarker CA72-4
作者： Dan Wu , Zhankui Guo , Yixin Liu , Aiping Guo , Wanruo Lou
DOI: 10.1016/J.TALANTA.2014.11.025
关键词:
摘要: Abstract A novel and sensitive nonenzymatic sandwich-type electrochemical immunosensor for the detection of gastric cancer biomarker CA72-4 was fabricated using dumbbell-like PtPd-Fe 3 O 4 nanoparticles (NPs) as a kind label. The signal amplification strategy, synergetic effect present in to increase reduction ability NPs toward H 2 , improved sensitivity immunosensor. constructed by modifying glassy carbon electrode with reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) effective immobilization primary anti-CA72-4 antibody (Ab 1 ). Secondary ) adsorbed onto NPs. proposed displayed wide linear range (0.001–10 U/mL) low limit (0.0003 U/mL). evaluated serum samples, receiving satisfactory results. Therefore, possesses excellent clinical value screening well convenient point-of-care diagnostics.
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