Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations.

摘要: For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements eye (cornea lens) serve objective for most systems, use lens, even 0 Dpt. power, can alter system's optical properties. In this investigation we examined effective focal length change aberrations that arise from lens. First, changes were simulated Zemax model. Then ocular without measured Shack-Hartmann wavefront sensor (SHWS) customized AO-SLO system. Total RMS errors two groups mice (14-month, 2.5-month-old), decomposed into 66 Zernike aberration terms, compared. These data revealed vertical coma spherical increased our Based on evaluated effect system performance function pupil size. Both error Strehl ratios quantified mice, lenses, different input beam sizes. results provide information determining optimum size adaptive optics, raise critical issues design systems incorporate lenses.