Nucleic acid detection system

作者: Youngsun Kim , Jaean Jung , Young Ho Choi

DOI:

关键词:

摘要: The present application discloses a system for detecting target nucleic acid comprising: container comprising amplification mix primer labeled with different haptens at its 5' and 3' ends, optionally dNTP hapten to form complex; lateral flow test device reservoir area reagent conditions suitable binding of specific partner the dye complex, wherein is linked or conjugated reporter another hapten; aspect complex.

参考文章(13)
Alan M. Nelson, Christian Bobritchi, Allan D. Pronovost, One-step fluorescent immunosensor test ,(1998)
Richard C. Ebersole, Sandra Fitzpatrick-McElligott, Edwin R. Hendrickson, William R. Majarian, Mark S. Payne, Jan A. Rafalski, Method for detection of non-denatured nucleic acid fragments ,(1997)
Stephen Quirk, Modular peptide-based reagent ,(2002)
Pascal Mertens, Ismaelle Renuart, Thierry Leclipteux, One step oligochromatographic device and method of use ,(2004)
Lopez Gemma Limones, De Sarabia Rosado Pedro Manuel Franco, Alberdi Maria Dolores Marin, Seiz Antonio Madejon, Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures ,(2002)
Gerald John Allen, Donald Leonard Nicholas Cardy, Lateral flow assay device and method ,(2003)
Diane L. Kozwich, Lynn D. Jankovsky, John C. Gerdes, Self-contained device integrating nucleic acid extraction, amplification and detection ,(1996)
Harri Härmä, Tero Soukka, Timo Lövgren, Europium Nanoparticles and Time-resolved Fluorescence for Ultrasensitive Detection of Prostate-specific Antigen Clinical Chemistry. ,vol. 47, pp. 561- 568 ,(2001) , 10.1093/CLINCHEM/47.3.561
Rune Björkman, Ib Mendel-Hartwig, Flow matrix assay device with movable separating member ,(2000)