Static DNA cytometry as a diagnostic aid in effusion cytology: II. DNA aneuploidy for identification of neoplastic cells in equivocal effusions

摘要: Objective The sensitivity of conventional cytology for identification neoplastic cells in effusions is unsatisfactory, about 58%. rate diagnostically equivocal routine 6%. DNA aneuploidy has previously been proven to be a sensitive and specific marker the tumor effusions. In present study we determined if malignancy can identified cytologically using as marker, thus decreasing diagnoses Study design One hundred serous cavities were obtained from diagnostic material. Nuclear content was measured after Feulgen staining TV image analysis system. Data correlated with patient follow-up. Results assumed abnormal stemlines, coefficient variation first stemline > or = 10% 9c observed. 55.9%. Specificity nonaneuploidy benignity 94.1%. positive predictive value occurrence malignant 97.9% since all but one aneuploid case showed Conclusion Image cytometry applying parameter able detect every second case. Thus, this method increase accuracy effusion by