Enhancer of Zeste 2 (EZH2) is up-regulated in malignant gliomas and in glioma stem-like cells.
作者： F. Orzan , S. Pellegatta , P. L. Poliani , F. Pisati , V. Caldera
DOI: 10.1111/J.1365-2990.2010.01132.X
关键词:
摘要: F. Orzan, S. Pellegatta, P. L. Poliani, Pisati, V. Caldera, Menghi, D. Kapetis, C. Marras, Schiffer and G. Finocchiaro (2011) Neuropathology Applied Neurobiology37, 381–394 Enhancer of Zeste 2 (EZH2) is up-regulated in malignant gliomas glioma stem-like cells Aims: Proteins the Polycomb repressive complex (PRC2) are epigenetic gene silencers involved tumour development. Their oncogenic function might be associated with their role stem cell maintenance. The histone methyltransferase a key member PRC2 function: we have investigated its expression gliomas. Methods:EZH2 was studied grade II–IV cells (GSC) by quantitative PCR immunohistochemistry. Effects EZH2 down-regulation were analysed treating GSC deacetylase (HDAC) inhibitor suberoylanide hydroxamic acid (SAHA) shRNA. Results: DNA microarray analysis showed that highly expressed murine human GSC. Real-time on different (n = 66) indicated more glioblastoma multiforme (GBM) than low-grade (P = 0.0013). This confirmed immunohistochemistry an independent set 106 Treatment SAHA caused significant up-regulation predicted target genes, disruption decreased marker CD133. Inhibition shRNA decrease proliferation. Conclusion: data suggest plays progression encourage therapeutic targeting these malignancies HDAC inhibitors.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(33)
Johan van der Vlag, Arie P. Otte, Transcriptional repression mediated by the human polycomb-group protein EED involves histone deacetylation. Nature Genetics. ,vol. 23, pp. 474- 478 ,(1999) , 10.1038/70602
Sarah E. Furchert, Claudia Lanvers-Kaminsky, Heribert Juürgens, Manfred Jung, Adele Loidl, Michael C. Frühwald, Inhibitors of histone deacetylases as potential therapeutic tools for high-risk embryonal tumors of the nervous system of childhood International Journal of Cancer. ,vol. 120, pp. 1787- 1794 ,(2007) , 10.1002/IJC.22401
Bradley E. Bernstein, Tarjei S. Mikkelsen, Xiaohui Xie, Michael Kamal, Dana J. Huebert, James Cuff, Ben Fry, Alex Meissner, Marius Wernig, Kathrin Plath, Rudolf Jaenisch, Alexandre Wagschal, Robert Feil, Stuart L. Schreiber, Eric S. Lander, A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells Cell. ,vol. 125, pp. 315- 326 ,(2006) , 10.1016/J.CELL.2006.02.041
Zhihua An, Christian B. Gluck, Megan L. Choy, Laura J. Kaufman, Suberoylanilide hydroxamic acid limits migration and invasion of glioma cells in two and three dimensional culture. Cancer Letters. ,vol. 292, pp. 215- 227 ,(2010) , 10.1016/J.CANLET.2009.12.006
Florian A Siebzehnrubl, Ina Jeske, Dorit Müller, Rolf Buslei, Roland Coras, Eric Hahnen, Hagen B Huttner, Denis Corbeil, Johanna Kaesbauer, Thomas Appl, Stephan Von Hörsten, Ingmar Blümcke, None, Spontaneous In Vitro Transformation of Adult Neural Precursors into Stem‐Like Cancer Cells Brain Pathology. ,vol. 19, pp. 399- 408 ,(2009) , 10.1111/J.1750-3639.2008.00189.X
Jeongwu Lee, Myung Jin Son, Kevin Woolard, Nicholas M. Donin, Aiguo Li, Chui H. Cheng, Svetlana Kotliarova, Yuri Kotliarov, Jennifer Walling, Susie Ahn, Misuk Kim, Mariam Totonchy, Thomas Cusack, Chibawanye Ene, Hilary Ma, Qin Su, Jean Claude Zenklusen, Wei Zhang, Dragan Maric, Howard A. Fine, Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell. ,vol. 13, pp. 69- 80 ,(2008) , 10.1016/J.CCR.2007.12.005
Sunit Das, Maya Srikanth, John A Kessler, Cancer stem cells and glioma Nature Reviews Neurology. ,vol. 4, pp. 427- 435 ,(2008) , 10.1038/NCPNEURO0862
Karl Agger, Paul A. C. Cloos, Jesper Christensen, Diego Pasini, Simon Rose, Juri Rappsilber, Irina Issaeva, Eli Canaani, Anna Elisabetta Salcini, Kristian Helin, UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature. ,vol. 449, pp. 731- 734 ,(2007) , 10.1038/NATURE06145
R. Cao, L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, Y. Zhang, Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing Science. ,vol. 298, pp. 1039- 1043 ,(2002) , 10.1126/SCIENCE.1076997
Jeongwu Lee, Svetlana Kotliarova, Yuri Kotliarov, Aiguo Li, Qin Su, Nicholas M. Donin, Sandra Pastorino, Benjamin W. Purow, Neil Christopher, Wei Zhang, John K. Park, Howard A. Fine, Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell. ,vol. 9, pp. 391- 403 ,(2006) , 10.1016/J.CCR.2006.03.030