Monoclonal Antibody Techniques Applied to Viruses

摘要: Publisher Summary A polyclonal serum against a given virus may have the ability to neutralize virus, fix complement, inhibit hemagglutination by or facilitate killing of virus-infected cells macrophages and lymphocytes. Monoclonal antibodies large number viruses already been generated, some them characterized as their biological properties. are useful in discerning structural functional properties various viral proteins instrumental identification antigenic variants that were not previously known. This chapter presents list representative which monoclonal including nature antigens used for immunization, assay detection antibodies, type obtained. It discusses principles hybridoma techniques with broad technical description. The general strategy immunizing animals, fusion protocol, methods antibody screening, characterization antigens, generation variants. also specific protocol preparing reagents.