Characterization of a novel differentiation antigen complex recognize by a monoclonal antibody (A-1A5): unique activation-specific molecular forms on stimulated T cells.

摘要: By using a single monoclonal antibody, novel glycoprotein complex composed of at least three distinct bands was defined on the surface mitogen- or alloantigen-stimulated T cells. These (210,000, 165,000, and 130,000 Mr) were not disulfide linked could be radio-labeled with 125I 35S-methionine readily detected by immunoprecipitation antibody A-1A5. Only approximately 20% normal cells (E rosette positive) 47% non-T negative) reactive However, upon activation cells, amount A-1A5 binding per cell percentage positive significantly increased. This increase most pronounced in activated subpopulations currently undergoing division (S, G2, M phases), which became 79% (after PHA stimulation) 99% (in long-term culture alloantigen IL2). Resting lymphocytes contained only Mr band The two other (210,000 165,000 markers for appeared several days after stimulation especially prominent addition exogenous IL 2. All lymphoblastoid lines tested expressed lower (130,000 Mr), line HSB also one activation-related larger proteins (165,000 Mr). B very weak U-937 monocyte-macrophage lineage) (145,000 aligned any found lymphoid estimated number sites much higher (11 X 10(5)), generally myeloid lineage (1 to 4 10(5)) than (0.2 1.3 10(5)).