Cellular Traction Stresses Increase with Increasing Metastatic Potential

摘要: Cancer cells exist in a mechanically and chemically heterogeneous microenvironment which undergoes dynamic changes throughout neoplastic progression. During metastasis, from primary tumor acquire characteristics that enable them to escape the migrate through stromal environment establish secondary tumors. Despite being linked poor prognosis, there are no direct clinical tests available diagnose likelihood of metastasis. Moreover, physical mechanisms employed by metastatic cancer poorly understood. Because metastasis most solid tumors requires exert force reorganize navigate dense stroma, we investigated differences cellular generation between non-metastatic cells. Using traction microscopy, found human breast, prostate lung cell lines, stresses were significantly increased compared counterparts. This trend was recapitulated isogenic MCF10AT series breast Our data also indicate matrix stiffness collagen density promote forces, generate higher forces than across all properties studied. Additionally, spreading for these lines has relationship with density, but biphasic substrate stiffness, indicating area alone does not dictate magnitude stress generation. Together, suggest contractile may play an important role regulate These findings critical understanding extracellular