Quantum-Dot-Based Lateral Flow Immunoassay for Detection of Neonicotinoid Residues in Tea Leaves
作者： Shuangjie Wang , Ying Liu , Shasha Jiao , Ying Zhao , Yirong Guo
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摘要: Neonicotinoid insecticides are commonly used for pest control on tea plantations as a result of their broad-spectrum activity. However, neonicotinoid residues released from leaves into infusions pose dietary risk to consumers. Therefore, rapid, sensitive, and reliable on-site detection method neonicotinoids is needed. We developed quantum-dot-based fluorescent lateral flow immunochromatographic strip (LFICS) combined with broad-specific antibody typical (imidacloprid, imidaclothiz, clothianidin), sensitivities [50% inhibitory concentration (IC50)] 0.104–0.33 ng/mL visual limits 0.5–1 ng/mL. The assay could be completed in less than 30 min. Using the LFICS analyze spiked samples (green tea, black oolong tea), average recovery three ranged between 71 111%, coefficient variation below 12%. results tests field were consistent resul...
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