人類Ste20蛋白質激酵素，Mst3，在細胞凋亡與OXPHOS系統調控機轉之研究

摘要: Mammalian sterile-20 protein kinase 3 (Mst3) plays an essential role in the apoptotic and non-apoptotic pathway. Caspases are shown to be involved Mst3-mediated apoptosis, but staurosporine-induced apoptosis is slightly suppressed by caspase inhibitor, z-DEVD-fmk. Mst3 though that not only involves caspase-dependent also caspase-independent The study demonstrates controls mitochondrial integrity through regulation of Bax subsequently promote nuclear translocation proapoptotic members, apoptosis-inducing factor endonuclease G from mitochondria HeLa cells. Following, nuclease activity associated with modulated. Thus, inferred participation mediated its cellular localization intermembrane space mitochondria. bold confirmed co-immunofluorescent staining, transmission electron microscopic imaging immuno-blotting after subcellular fractionation. Similar results observed human trophoblast cell line 3A-sub-E cells, suggesting a general phenomenon. targeting segment between residues 314-431. space-Mst3 regulate respiratory transport chain complexes I III dependence glucose for ATP synthesis. indicate important dependent independent- pathway oxidative phosphorylation.