Leucine and protein metabolism after bilateral nephrectomy in rats: the role of hepatic tissue.
作者： Tilser I , Holecek M , Sprongl L , Tichý M
DOI:
关键词:
摘要: The aim of this study was to evaluate the effect acute uremia on changes in leucine and protein metabolism whole body hepatic tissue. Acute renal insufficiency induced by bilateral nephrectomy (BNX). Twenty-four hours later, parameters amino acid were evaluated using primed constant intravenous infusion L-[1-14C]leucine, isolated perfused liver (IPL) cx-keto[1-14C]isocaproate. control group consisted sham-operated rats. BNX a marked decrease proteolysis, synthesis, oxidized fraction clearance. synthesis higher than proteolysis. A significant drop observed muscle, gut, heart spleen. with IPL animals showed decreased oxidation ketoisocaproic concentrations branched-chain acids (BCAA) leucine, isoleucine valine perfusion solution. We conclude that cause rapid depletion proteins after is greater proteolysis associated an increase fraction. data obtained model indicate causes metabolic enable resynthesis BCAA from corresponding keto liver.
参考文章(21)
Robert T. Taylor, W. Terry Jenkins, Leucine Aminotransferase II. PURIFICATION AND CHARACTERIZATION Journal of Biological Chemistry. ,vol. 241, pp. 4396- 4405 ,(1966) , 10.1016/S0021-9258(18)99734-6
T B Patel, M S DeBuysere, L L Barron, M S Olson, Studies on the regulation of the branched chain alpha-keto acid dehydrogenase in the perfused rat liver. Journal of Biological Chemistry. ,vol. 256, pp. 9009- 9015 ,(1979) , 10.1016/S0021-9258(19)52500-5
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
R. C. May, Y. Hara, R. A. Kelly, K. P. Block, M. G. Buse, W. E. Mitch, Branched-chain amino acid metabolism in rat muscle: abnormal regulation in acidosis American Journal of Physiology-endocrinology and Metabolism. ,vol. 252, ,(1987) , 10.1152/AJPENDO.1987.252.6.E712
P. J. Garlick, D. J. Millward, W. P. T. James, The diurnal response of muscle and liver protein synthesis in vivo in meal-fed rats Biochemical Journal. ,vol. 136, pp. 935- 945 ,(1973) , 10.1042/BJ1360935
B K England, J L Bailey, R Caban, D Reaich, W E Mitch, S R Price, A C Marinovic, B J Maroni, Mechanisms contributing to muscle-wasting in acute uremia: activation of amino acid catabolism. Journal of The American Society of Nephrology. ,vol. 9, pp. 439- 443 ,(1998) , 10.1681/ASN.V93439
Milan Holeček, Ivan Tilšer, Frantis̄ek Skopec, Ludēk Šprongl, Leucine metabolism in rats with cirrhosis Journal of Hepatology. ,vol. 24, pp. 209- 216 ,(1996) , 10.1016/S0168-8278(96)80031-6
William E. Mitch, Uremic acidosis and protein metabolism. Current Opinion in Nephrology and Hypertension. ,vol. 4, pp. 488- 492 ,(1995) , 10.1097/00041552-199511000-00005
Yoko Hara, Robert C. May, Ralph A. Kelly, William E. Mitch, Acidosis, not azotemia, stimulates branched-chain, amino acid catabolism in uremic rats. Kidney International. ,vol. 32, pp. 808- 814 ,(1987) , 10.1038/KI.1987.280
Stefano Del Canale, Enrico Fiaccadori, Nicoletta Ronda, Karin Söderlund, Carla Antonucci, Achille Guariglia, Muscle energy metabolism in uremia. Metabolism-clinical and Experimental. ,vol. 35, pp. 981- 983 ,(1986) , 10.1016/0026-0495(86)90032-6