A study of complement fixation by rheumatoid factor using a haemolytic assay system.
作者： J B Natvig , P M Johnson , O J Mellbye , M M Taylor-Upsahl
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摘要: Complement fixation by rheumatoid factor (RF) in sera from arthritis patients has been investigated means of a haemolytic assay system employing sheep erythrocytes (SRBC) coated with reduced and alkylated rabbit IgG anti-SRBC antibody. Haemolysis was almost invariably detected RF-positive whereas haemolysis not observed RF-negative sera; It evident marked increase haemolysis, obtained following isolation IgM-RF high purity several sera, that the full vitro complement-fixing ability hence lytic potential RF serum is masked. Parallel observations were also made synovial fluids. The inhibition fluids commonly involved reduction degree lysis at concentration test material as well more generalized overall percentage lysis. Complete replication typical fluid patterns on dilution achieved addition heat-aggregated human to isolated preparations demonstrated two inhibitory effects are separable terms complement depletion free antibody activity, respectively.
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