An analysis of the proteomic profile for Thermoanaerobacter tengcongensis under optimal culture conditions.
作者： Jingqiang Wang , Yanfen Xue , Xiaoli Feng , Xiaolei Li , Hao Wang
关键词:
摘要: The genome of Thermoanaerobacter tengcongensis is estimated to encode 2588 theoretical proteins. In this study, we have vitalized approximately 46% the proteome experimentally using a proteomic strategy that combines three different methods, shotgun digestion plus high-performance liquid chromatography (HPLC) with ion-trap tandem mass spectrometry (shotgun-liquid (LC)/MS), one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) HPLC (one-dimensional (1DE)-LC/MS), and two-dimensional matrix-assisted laser desorption/ionization-time flight-mass (2DE-MALDI-TOF-MS). Of 1200 proteins identified, as few 76 were globally found by all approaches, notably, most these in soluble fraction. However, there number unique detected one method only, suggesting our provides means toward obtaining comprehensive view protein expression profile. Proteins from major metabolic pathways are strongly represented on map, enzymes identified more than method. Based upon present able broaden understanding how T. survives under high temperature environment, whereas several its properties can not be fully explained data.
sci-hub.se 参考文章(36)
Michael J MacCoss, Christine C Wu, Shotgun proteomics: tools for the analysis of complex biological systems. Current Opinion in Molecular Therapeutics. ,vol. 4, pp. 242- 250 ,(2002)
Boguslaw Stec, Mary F. Roberts, Hongying Yang, Kenneth A. Johnson, Liangjing Chen, MJ0109 is an enzyme that is both an inositol monophosphatase and the 'missing' archaeal fructose-1,6-bisphosphatase. Nature Structural & Molecular Biology. ,vol. 7, pp. 1046- 1050 ,(2000) , 10.1038/80968
Keiichi Fukuyama, Yoshitomo Nagahara, Tomitake Tsukihara, Yukiteru Katsube, Toshiharu Hase, Hiroshi Matsubara, Tertiary structure of Bacillus thermoproteolyticus [4Fe-4S] ferredoxin Journal of Molecular Biology. ,vol. 199, pp. 183- 193 ,(1988) , 10.1016/0022-2836(88)90388-9
Michael P. Washburn, Dirk Wolters, John R. Yates, Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nature Biotechnology. ,vol. 19, pp. 242- 247 ,(2001) , 10.1038/85686
Andreas Ruepp, Werner Graml, Martha-Leticia Santos-Martinez, Kristin K. Koretke, Craig Volker, H. Werner Mewes, Dmitrij Frishman, Susanne Stocker, Andrei N. Lupas, Wolfgang Baumeister, The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum Nature. ,vol. 407, pp. 508- 513 ,(2000) , 10.1038/35035069
David J DeRosier, The Turn of the Screw: The Bacterial Flagellar Motor Cell. ,vol. 93, pp. 17- 20 ,(1998) , 10.1016/S0092-8674(00)81141-1
Yonglin Hu, Salem Faham, Roopali Roy, Michael W.W Adams, Douglas C Rees, Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus: the 1.85 A resolution crystal structure and its mechanistic implications. Journal of Molecular Biology. ,vol. 286, pp. 899- 914 ,(1999) , 10.1006/JMBI.1998.2488
Thomas Hansen, Peter Schönheit, Purification and properties of the first-identified, archaeal, ATP-dependent 6-phosphofructokinase, an extremely thermophilic non-allosteric enzyme, from the hyperthermophile Desulfurococcus amylolyticus. Archives of Microbiology. ,vol. 173, pp. 103- 109 ,(2000) , 10.1007/S002039900114
Volker Neuhoff, Reinhard Stamm, Hansjörg Eibl, Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis Electrophoresis. ,vol. 6, pp. 427- 448 ,(1985) , 10.1002/ELPS.1150060905
Jochen Heukeshoven, Rudolf Dernick, Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining Electrophoresis. ,vol. 6, pp. 103- 112 ,(1985) , 10.1002/ELPS.1150060302