Quantitative Analysis of Drug Efficacy on C. elegans Models for Neuromuscular Diseases

摘要: Caenorhabditis elegans has emerged as a powerful model organism for drug screening due to its cellular simplicity, genetic amenability and homology humans combined with small size low cost. Currently, high-throughput assays are mostly based on image-based phenotyping not exploiting key locomotory parameters of this multicellular muscles such thrashing force, critical parameter when drugs muscle-related diseases. In study, we demonstrated the use micropillar-based force assay chip in combination an imaging evaluate efficacy various currently used treatment neuromuscular Using two-dimensional approach, showed that was generally more sensitive measuring Duchenne Muscular Dystrophy Parkinson’s Disease mutant worms well partly Amyotrophic Lateral Sclerosis model. These results underline potential our candidates diseases analysis approach.